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 titi_marinoxx|T I T I • M A R I N O @Titi Barfoed’s test makes use of Barfoed’s solution, which contains copper acetate in the dilute acetic acid with a pH of 4.6. Principle : In Barfoed’s test, the reducing monosaccharide is oxidized by the copper ion in the solution to form a carboxylic acid and copper (I) oxide, which results in the formation of a red-coloured precipitate.

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